Sains Malaysiana 53(7)(2024): 1617-1630

http://doi.org/10.17576/jsm-2024-5307-11

 

Antioxidant Activities and Laxative Effect of Bioactive Compounds from Cynara cardunculusvar. sylvestris

(Aktiviti Antioksidan dan Kesan Laksatif Sebatian Bioaktif daripada Cynara cardunculusvar. sylvestris)

 

FATMA TAJINI*, AWATEF JELASSI, AMAL HAMDANI, ALI SALEM, OLA ABDELHEDI, ABID OUERGHUI & HICHEM SEBAI

 

Laboratory of Functional Physiology and Valorization of Bio-ResourcesHigher Institute of Biotechnology of Béja, University of Jendouba, Avenue Habib Bourguiba, B.P. 382, ​​9000 Béja, Tunisia

 

Received: 19 January 2024/Accepted: 4 June 2024

 

Abstract

The present study aims to investigate the antioxidant and anti-constipation activities of the derived phenolic extracts in ribs wild cardoon (Cynara sylvestris). Analysed extracts (acetone, methanol and aqueous) exhibited high level of phenolic compounds and excellent antioxidant activities with significant IC50 values (p ≤0.05), as assessed by the DPPH radical-scavenging activity (0.84±0.08 - 2.44±0.007 μg/mL), ABTS cationic radical test (0.96 ±0.01- 136.67±6.75 μg/mL) and β-carotene bleaching assay (2.09±0.02-51.12±1.32 μg/mL). Also, higher levels of insoluble dietary fibers were found (56.18 ± 0.91% DW of neutral detergent fiber). The in vivo investigation was performed on Wistar rats to explore the ability of C. sylvestris aqueous extract (CSAE) in the enhancement of the gastrointestinal transit and the treatment of induced constipation by Loperamide (commercialized as Idium). A significant increase of 15, 24.5 and 32.4% in gastrointestinal motility was recordedwhen doses of CSAE increased (75, 150 and 300 mg/kg b.w, respectively). Food intake, water consumption, number and weight of stools were also increased in a dose-dependent manner, compared to the positive control (untreated). Moreover, CSAE provided significantly (p ≤0.05) and dose-dependently protection against oxidative stress by preserving normal antioxidant enzymes activities (SOD and GPx) in intestinal and colonic mucosa, and resorted hepatic enzymes (AST and ALT) and renal (urea and creatinine) levels to normal values. These results can be explained by the abundance of phenolic compounds and insoluble fibers in this plant. Therefore, its use can be encouraged in alimentary and pharmaceutical applications as antioxidant and laxative food supplements.

 

Keywords: Anti-constipation; Cynara cardunculus var. sylvestris; gastrointestinal motility; oxidative stress; phenolic compounds

 

Abstrak

Penyelidikan ini bertujuan untuk mengkaji aktiviti antioksidan dan anti-sembelit daripada ekstrak fenol yang diperoleh dalam kadun liar rusuk (Cynara sylvestris). Ekstrak yang dianalisis (aseton, metanol dan akueus) menunjukkan tahap sebatian fenol yang tinggi dan aktiviti antioksidan yang sangat baik dengan nilai IC50 yang ketara (p≤0.05), seperti yang dinilai oleh aktiviti penangkapan radikal DPPH (0.84±0.08 - 2.44±0.007 μg/mL), ujian radikal kationik ABTS (0.96 ±0.01- 136.67±6.75 μg/mL) dan ujian pelunturan β-karotena (2.09±0.02-51.12±1.32 μg/mL). Juga, paras serat makanan tidak larut yang lebih tinggi didapati (56.18 ± 0.91% DW serat detergen neutral). Penyelidikanin vivo telah dijalankan ke atas tikus Wistar untuk mengkaji keupayaan ekstrak akueus C. sylvestris (CSAE) dalampeningkatan transit gastro usus dan rawatan sembelit yang disebabkan oleh Loperamide (dikomersialkan sebagai Idium). Peningkatan ketara sebanyak 15, 24.5 dan 32.4% dalam motiliti gastro usus direkodkan apabila dos CSAE meningkat (masing-masing 75, 150 dan 300 mg/kg b.w). Pengambilan makanan, penggunaan air, bilangan dan berat najis juga dinaikkan secara kebergantungan dos, berbanding kawalan positif (tidak dirawat). Tambahan pula CSAE memberikan perlindungan yang ketara (p≤0.05) dan kebergantungan dos terhadap tekanan oksidatif dengan mengekalkan aktiviti enzim antioksidan normal (SOD dan GPx) dalam usus dan kolon mukosa dan menjadikan tahap enzim hepatik (AST dan ALT) dan buah pinggang (urea dan kreatinin) kepada nilai normal. Keputusan ini boleh dijelaskan oleh banyaknya sebatian fenol dan gentian tidak larut dalam tumbuhan ini. Oleh itu, penggunaannya boleh digalakkan dalam pengaplikasian makanan dan farmaseutikal sebagai makanan tambahan antioksidan dan laksatif.

 

Kata kunci: Anti-sembelit; Cynara cardunculus var. sylvestris; motiliti gastro usus; sebatian fenol; tekanan oksidatif

 

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*Corresponding author; email: tajinifatma24@gmail.com

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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